Pluripotency

In the Merrill lab, we endeavor to reveal fundamental molecular mechanisms driving cell fate decisions, and how these decisions direct embryogenesis and stem cell maintenance in vivo. We center our efforts on the roles of a family of DNA-binding transcriptional regulators called Tcf/Lef factors. Tcf/Lef proteins can either activate or repress transcription of target genes depending upon the cohort of physically interacting proteins available to interact with a Tcf/Lef protein within a given cell. We are interested in understanding how these biochemical interactions determine cell fates in the intact mouse, and we use powerful techniques of mouse molecular genetics to elucidate the roles of Tcf factors in a variety of embryonic and postnatal contexts involving stem cells.

Current research is focusing on the roles of Tcf factors in pluripotent stem cells in the embryo during the process of gastrulation. In addition to this basic research on stem cells, we have a strong interest in cancer. In particular, we have been intrigued by observations that Tcf factors (and the upstream Wnt signaling pathway) promote self-renewal of stem cells and the formation of tumors in several different adult organs. Based on this interest, an underlying role for Tcf factors in regulating stem cell proliferation as it relates to cancer is being examined. The function of Tcf factors, particularly Tcf3, in the morphogenesis of several organs during mouse embryogenesis is a third area that the lab is pursuing. This avenue of research is supported by phenotypes of mice lacking the Tcf3 protein or lacking the ability of Tcf3 to interact with beta-catenin.

The lab is currently looking for highly motivated and qualified postdoctoral researchers interested in discovering the function of Tcf factors in in vivo contexts in the mouse. In particular we are looking for postdocs with an interest in the vasculature, limb formation, the prostate, and the gastrointestinal tract. If you have an interest in joining an enthusiastic and fun team dedicated to scientific discovery involving biochemical determinants of transcriptional regulation of embryonic development, contact Dr. Merrill.